Donkey Kong Country 2: Diddy's Kong Quest is the second installment in the Donkey Kong Country series released in 1995. It was developed by Rareware and published by Nintendo for the Super Nintendo Entertainment System. It is a sequel and direct follow-up of Donkey Kong Country. The subtitle was originally Diddy Kong's Quest, but it was later changed to Diddy's Kong Quest, the latter two words being a pun on "conquest." The game stars Diddy Kong, as he and his girlfriend, Dixie Kong, make their way through Crocodile Isle to save Donkey Kong from the Kremling Krew. A sequel to Donkey Kong Country 2: Diddy's Kong Quest was released in 1996, Donkey Kong Country 3: Dixie Kong's Double Trouble!

In 2004, a remake of the game was made for the Game Boy Advance. It features several differences, including the addition of photographs and extra Cranky's Video Game Hero Coins. It omits the Diddy's Kong Quest subtitle, thus the game is simply known as Donkey Kong Country 2.

In 2007, the original version was re-released on the Wii's Virtual Console. On November 25, 2012, for reasons unknown, Donkey Kong Country 2 and the other two original Donkey Kong Country games were delisted from the Wii Virtual Console, but on October 30, 2014, the games were relisted in Europe and Australia. Around the same time, the games were released for the Wii U's Virtual Console in Europe and Australia, in Japan on November 26, 2014, and in the United States and Canada on February 26, 2015. For handhelds, Donkey Kong Country 2: Diddy's Kong Quest was ported exclusively to the New Nintendo 3DS's Virtual Console in March/April 2016. It was made available on Super Nintendo Entertainment System - Nintendo Switch Online for the Nintendo Switch on September 23, 2020.

Donkey Kong Country 2: Diddy's Kong Quest is the sixth best-selling Super Nintendo Entertainment System game, with its predecessor being the third. A similar Game Boy title was released a year later, in 1996, Donkey Kong Land 2.