Second Samurai is a video game. It is the sequel to the 1991 game First Samurai, released for the Amiga in 1993 and ported to the Mega Drive in 1994.

The game includes prehistoric and futuristic levels as well as feudal Japan as in First Samurai. Contact with enemies or falling off the screen (you do not lose a life automatically) subtracts one point from your life bar. In the Mega Drive/Genesis version of the game, the Demon King from the first game taunts you on a black screen when you lose a life with sayings such as "Give up mortal!" and "Going Down!" until you lose your last life.