Yu-Gi-Oh! The Eternal Duelist Soul is a video game for the Game Boy Advance. It is an English-language version of Yu-Gi-Oh! Duel Monsters 5 Expert 1 but is modified with the graphics and music of Yu-Gi-Oh! Duel Monsters 6 Expert 2.

A red, green, or black deck is automatically available at the start of the game. Opponents are arranged in "tiers", which are unlocked by defeating each opponent in the previous tier a certain number of times. Except for Tier 5, which has 4 duelists, each tier consists of 5 duelists.

There are 819 different cards in total. Cards are accessed from the Deck Edit menu and are stored in the Trunk (which can hold more than 10,000 cards). They can be transferred to the Main Deck or Side Deck. The Main Deck must contain 40-60 cards, but also allows up to an additional 20 cards in the Fusion Deck section. The Side Deck can hold up to 15 cards, which becomes available between certain special event duels, such as Weekend Duels.

An important aspect of the game is the in-game calendar. On certain days of the week, random events may occur, such as receiving a pack of cards in the mail or being challenged to a Match by an opponent. The calendar advances one day after each duel.

The Eternal Duelist Soul is nearly identical to Stairway to the Destined Duel, except there are fewer cards to choose from and the default animation speed is slower, though there is the option of holding the R button before the duel starts to permanently speed up that particular duel. It is also the first game to include Polymerization as an actual card.