Dr. Mario & Panel de Pon, known as Dr. Mario & Puzzle League outside of Japan is the 2nd compilation in the Panel de Pon series, the first one was in the Nintendo Puzzle Collection. This game uses the engine in the Multiboot rom of Panel de Pon seen in Nintendo Puzzle Collection, but with added features such as Vs. mode, Stage Clear, & Puzzle Mode.

This is the first game to introduce the character-less marketed rebranding of the series, which would follow into the release of Panel de Pon DS, further diverting its identity away from previous games.