PGA Tour Golf features three real golf courses: PGA West, TPC Avenel, and TPC Sawgrass. It also includes a fictional fourth course, Sterling Shores. Various game modes are featured, including Tournament, Driving, Putting, and Practice. In Tournament, the player competes against a total of 60 unseen players, whose statistics are displayed on a scoreboard. The player can watch a replay of any golf shot, and good shots are automatically replayed. The game includes variable wind conditions which affect where the ball will land.

Before starting each course, the player is given a three-dimensional view of the hole, and the camera pans from the hole to the player's starting position. To further aid the player, an overhead map of the course is shown before starting each hole and after each shot.[5] Upon reaching the green, the player views the area as a three-dimensional contoured grid to study the uneven terrain for better putting. The player also receives advice from PGA golfers. The Amiga and Master System versions include a multiplayer option. A 1994 U.K. re-release of the Amiga version includes the three courses from PGA Tour Golf II, as well as three additional tournaments.